Hello, it would be useful to see how the merging of the normal data looks in addition to the anomalous, which could be a bit better, I fear. Are you expecting significant anomalous differences e.g. Se-Met, S with long wavelength, H-atom, etc? You should have quite high redundancy with all that data even with monoclinic. HTH a bit. Jon Cooper
Sent from ProtonMail mobile -------- Original Message -------- On 4 Nov 2020, 16:30, Almudena Ponce Salvatierra wrote: > Hello everyone, > > I have collected 6 small datasets from a crystal, each from a different point > on the crystal to avoid radiation damage, and there is some overlap among > them, as follows: > > - dts1: 0-90 degrees > - dts2: 60-150 degrees > - dts3: 120-210 degrees > - dts4: 180-270 degrees > - dts5: 240-330 degrees > - dts6: 300-390 degrees > > Diffraction is very poor and, after processing, I've cut resolution at around > 5 Angstroms for all of them. The space group is C2. Feeding the 6 of them to > scale_and_merge in Phenix gives the attached output. Does anybody who has > merged such low-resolution anomalous data before know whether this is a > "good" indicator (meaning there's a tiny light at the end of the tunnel) or > rather not? > > Any suggestions? (besides the obvious "go back to the lab and grow new > crystals") > > My aim is to combine these phases with a partial molecular replacement > solution and a native dataset at 3.5 Angstroms. Hopefully, then I would be > able to finish building the model. > > Thanks a tone in advance! > > Best, > > Almudena > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
