Hi Almudena, did you cut the res. after the merge of your "individual" datasets ? or you cut for each and then you merged? Because I will probably go for the first, I merge and then I decide where to cut. If you cut for each, maybe you "miss" the gain provide by the redundancy you have cumulated using all the data.
If I mis understood what you did, and return a bad answer, sorry. And to really answer your question, I will say : I stick to a combination of the "standard" stats, CC 1/2 mainly, I/sig and if you expect anomalous, CC Ano and SigAno. My choice would be conditioned also by the strategy for the phasing. Hope to help. De: "Almudena Ponce Salvatierra" <[email protected]> À: "CCP4BB" <[email protected]> Envoyé: Mercredi 4 Novembre 2020 17:30:29 Objet: [ccp4bb] scale and merge suggestions Hello everyone, I have collected 6 small datasets from a crystal, each from a different point on the crystal to avoid radiation damage, and there is some overlap among them, as follows: - dts1: 0-90 degrees - dts2: 60-150 degrees - dts3: 120-210 degrees - dts4: 180-270 degrees - dts5: 240-330 degrees - dts6: 300-390 degrees Diffraction is very poor and, after processing, I've cut resolution at around 5 Angstroms for all of them. The space group is C2. Feeding the 6 of them to scale_and_merge in Phenix gives the attached output. Does anybody who has merged such low-resolution anomalous data before know whether this is a "good" indicator (meaning there's a tiny light at the end of the tunnel) or rather not? Any suggestions? (besides the obvious "go back to the lab and grow new crystals") My aim is to combine these phases with a partial molecular replacement solution and a native dataset at 3.5 Angstroms. Hopefully, then I would be able to finish building the model. Thanks a tone in advance! Best, Almudena To unsubscribe from the CCP4BB list, click the following link: [ https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 | https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
