Probably a good idea to share an image :) worth many words... Artem
On Wed, Dec 9, 2020, 9:17 AM <MJ Xie> <sz20203020...@cau.edu.cn> wrote: > Dear All > There is a 43kd protein purified via Ni-chelating affinity > chromatography, anion exchange chromatography and gel filtration > chromatography in sequence. However the chromatogram obtained showed an > extremely asymmetric peak shape. The aggregation forms of proteins are > mainly in the range of monomers and dimers(Hepes and low concentration of > salt were used as buffers for gel filtration chromatography). 5% glycerinum > and 1mM Benzamidine hydrochloride had been added in order to maintain the > stability of the protein and prevent it from degrading. But well, all the > efforts seem to be useless. We wonder if there are any effective measures > can be taken to radically solve this problem. We would be much indebted for > the suggestions you offer. > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
