Exactly. Without a picture of SEC profile it's even difficult to decipher the meaning of "extremely asymmetric peak shape". Beside that, one domain or multi-domain protein, extended N-term or C-term loops containing constructs, column, load (weight & vol) and flow rate - any one of these could affect separation efficiency and/or peak characteristics. Regards,Reza
“The rule for web content is to keep it short. The rule for email content is to keep it ultra-short.” — Jakob Nielsen, ‘king of usability’ ____________________________ Md Rezaul Karim, Ph.D. Postdoctoral Research Fellow Department of Drug Discovery H. Lee Moffitt Cancer Center and Research Institute Tampa, FL 33612 Email: reza.ka...@moffitt.org, rez...@usf.edu Phone: (813) 745 4673 ext. 5462 https://orcid.org/0000-0002-0424-127X On Wed, Dec 9, 2020 at 11:42 AM, Artem Evdokimov<artem.evdoki...@gmail.com> wrote: Probably a good idea to share an image :) worth many words... Artem On Wed, Dec 9, 2020, 9:17 AM <MJ Xie> <sz20203020...@cau.edu.cn> wrote: Dear All There is a 43kd protein purified via Ni-chelating affinity chromatography, anion exchange chromatography and gel filtration chromatography in sequence. However the chromatogram obtained showed an extremely asymmetric peak shape. The aggregation forms of proteins are mainly in the range of monomers and dimers(Hepes and low concentration of salt were used as buffers for gel filtration chromatography). 5% glycerinum and 1mM Benzamidine hydrochloride had been added in order to maintain the stability of the protein and prevent it from degrading. But well, all the efforts seem to be useless. We wonder if there are any effective measures can be taken to radically solve this problem. We would be much indebted for the suggestions you offer. To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
