Dear Afshan, It is not totally clear to me how you did the merging, however, I have good experiences with that particular residue KCX in refinement. I used the Replace residue functionality of COOT described here: https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/docs/coot.html#Mutating-to-a-Non_002dStandard-Residue
Kind regards, Bjarte Aarmo Lund Postdoc UiT The Arctic University of Norway From: CCP4 bulletin board <[email protected]> On Behalf Of Afshan Begum Sent: Monday, February 8, 2021 14:46 To: [email protected] Subject: [ccp4bb] Fixing Modified residue Dear Expert, I have a trouble to fix the lysin modified residue in to the PDB coordinate. what i did, I get the KCX(Modified lysin) coordinate from monomer library in COOT (0.9) , merge in to the coordinate file , its listed into position 193 lysin then run rafmac 5 , its place the residue but as unconnected and produce its own number. In the second step I take the kcx coordinate in the text pdb file and place into between 192 and 194 and change reside number 193 . Save it. Now it can not be read for further refinement. Could you people suggest me how to fix it? Would be highly thankful to you. Best Regards =============================== Afshan ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
