Hi Saif, If your goal is to perform co-crystallization, I am completely agreed with David's suggestions. Delta Tm does matter in the co-crystallization but that's not always the case. I have some experience with protein complexes where I successfully co-crystallized by using really high molar ratio of substrate (1: 20, 1: 50, and even 1:200 given your substrate is not too much expensive). Try some additives relevant to your system.
Good luck Prem On Thu, 25 Feb 2021, 08:56 Saif Mohd, <[email protected]> wrote: > Hello everyone, > > 1) How much change in Tm (ΔTm) in a thermal shift assay is considered to > be significant ? > > 2) A negative ΔTm infers that the compound is making the protein > unstable. In such a case, will the co-crystallization be difficult or just > impossible or on the contrary it shouldn't matter much? > > > Thanks and best regards, > Saif > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
