Thank you everyone for the detailed explanations/suggestions and sharing your successful experience. It is very helpful.
Earlier I had thought that maybe with ΔTm, I could select the most promising molecules. But now it is unlikely the case. I should have also mentioned that the IC50 values for all the compounds are between 1-5uM. Thanks again, Have a wonderful weekend, Saif On Thu, Feb 25, 2021 at 7:38 PM Maria Cristina Nonato <[email protected]> wrote: > *Dear Saif* > > *Hope you are doing well and safe!* > > 1) How much change in Tm (ΔTm) in a thermal shift assay is considered to > be significant ? > > *As it has already been mentioned there is no specific cutoff for deltaTm > to be considered significant. DeltaTm depends on many factors, including > the type of dye you use, protein structure and where your compound binds*. > > > 2) A negative ΔTm infers that the compound is making the protein > unstable. In such a case, will the co-crystallization be difficult or just > impossible or on the contrary it shouldn't matter much? > > > *A negative detaTm means the compound is binding to a different > conformational state of the protein, compared to the native one. I would > not consider co-crystallization more difficult or impossible in the > presence of those compounds, but I would definitely screen for different > crystallization conditions.* > > *Good luck* > > *Cristy* > *#womeninscience* > > > Em qui., 25 de fev. de 2021 às 11:56, Saif Mohd <[email protected]> > escreveu: > >> Hello everyone, >> >> 1) How much change in Tm (ΔTm) in a thermal shift assay is considered to >> be significant ? >> >> 2) A negative ΔTm infers that the compound is making the protein >> unstable. In such a case, will the co-crystallization be difficult or just >> impossible or on the contrary it shouldn't matter much? >> >> >> Thanks and best regards, >> Saif >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
