Hi Herman, I will add a few points to all the excellent advice already provided to you. If you decide to try this option of crystallizing a fusion protein of your target, I would consider using N-terminal but also C-terminal fusions. We have had success using MBP in N-ter and the superfolder GFP in C-terminal for a few 'vexing' proteins from *Plasmodium falciparum. *It helped us either to obtain crystals/structures (as you seek) but also provided a way to circumvent a tendency for twinning from our target in some specific cases. GFP has the added benefit that your fusion crystals should be bright yellow so that speeds up the screening process a bit.
I hope this helps. Good luck Best regards, Pascal Egea, PhD UCLA School of Medicine > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <http://mednet.ucla.edu> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
