If you were lucky the new crystal might have the same cell and spacegroup as your model, but otherwiseThis is a case for molecular replacement.?
My course of action using CCP4I2 Process data carefully and look for any warnings. Align your new sequence with the model using clustalw Edit the model to have the new sequence - SCULTOR does this If the cells are the same you can just start refinement against the new data with the model in the same position . But if not Run PHASER or MOLREP to find the new orientation for the model. Now is the really difficult part - refining and rebuilding a structure with low resolution data. Maybe best to look for a better crystal! Eleanor. On Fri, 23 Apr 2021 at 20:26, Swati Gupta <visvasw...@gmail.com> wrote: > Dear all, > how do I proceed with solving a protein structure with 3.9 > A resolution and an i/sig i of 1.3 and cchalf of 0.8. The homologous > protein has 48% identity to be used as template. Kindly help with what > programs to run. > > > Thanks > Swati > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
