Hi Swati,
I am very far from expert, but if you have anisotropic diffraction have
you thought of bringing in Staraniso - public server at
https://staraniso.globalphasing.org? That should help keeping
reflections with higher I/sigma and rejecting those with mostly noise.
Yours,
Rasmus
On 23/04/2021 21:11, Swati Gupta wrote:
My data is moderate anisotropic also with a high Wilson b factor greater
than 200
On Sat, 24 Apr, 2021, 01:19 Eleanor Dodson, <[email protected]
<mailto:[email protected]>> wrote:
If you were lucky the new crystal might have the same cell and
spacegroup as your model, but otherwiseThis is a case for molecular
replacement.?
My course of action using CCP4I2
Process data carefully and look for any warnings.
Align your new sequence with the model using clustalw
Edit the model to have the new sequence - SCULTOR does this
If the cells are the same you can just start refinement against the
new data with the model in the same position .
But if not
Run PHASER or MOLREP to find the new orientation for the model.
Now is the really difficult part - refining and rebuilding a
structure with low resolution data.
Maybe best to look for a better crystal!
Eleanor.
On Fri, 23 Apr 2021 at 20:26, Swati Gupta <[email protected]
<mailto:[email protected]>> wrote:
Dear all,
how do I proceed with solving a protein
structure with 3.9 A resolution and an i/sig i of 1.3 and cchalf
of 0.8. The homologous protein has 48% identity to be used as
template. Kindly help with what programs to run.
Thanks
Swati
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Rasmus H. Fogh Tel.: +44 (0)1223 353033
Global Phasing Ltd., Fax.: +44 (0)1223 366889
Sheraton House,
Castle Park,
Cambridge CB3 0AX
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