Dear Randy, Eleanor and Jon,
Thank you for your kind rely. I apologize for about the tutorial files. I actually got it from my institution's sever and did not check the link. Thanks to Randy we now have the correct link. To John: I did put the correct domain 1 chain A and domain 2 chain B as fixed model but it seems domain 2 chain A is extra hard to be added in the third MR. To Randy: Your approach solved many questions in my mind on that misplaced domain. I tried single job with phaser before but it failed too; at the same time, I actually tried to put the domains 1 or domain 3 as the third domain in Molrep (also failed). Maybe I should inspect more in the map when putting domain 3. I also found the misplaced second domain one was in completely wrong orientation that the N-terminus and C-terminus shall be switched. Although I got quiet heave work load this week, I will definitely try your steps later. I cannot be more grateful for your help, especially there are limited learning materials online. Thanks again. best, Lande ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
