On Mon, 2021-06-07 at 01:35 +0530, vivek sharma wrote: > > How does one validate if 2 atoms (non-hydrogen) which are close enough, > actually participate in H-bonding? > I have a recent data that i am currently working on, the NH2 of ARG is at > 2.61A from ligand's oxygen atom (refer to > attached image), since there is no electron density for hydrogens, how can i > be sure if i am modelling ligand > correctly? >
This doesn't answer your question, but the difference map suggests radiation damage to the very site for which you require optimal clarity. Less dose, more crystals? Paul. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
