There are tools such as hbplus

https://www.ebi.ac.uk/thornton-srv/software/HBPLUS/

that will calculate predicted hydrogen bonds (including ligands, you just
need to define their chemistry) throughout a structure. These tools usually
go beyond simple distance considerations and analyze further geometry
details.

Proper donor acceptor pair at 2.6A is almost without doubt a hydrogen bond.

On Sun, Jun 6, 2021, 4:17 PM vivek sharma <[email protected]> wrote:

> Dear all,
>
> How does one validate if 2 atoms (non-hydrogen) which are close enough,
> actually participate in H-bonding?
> I have a recent data that i am currently working on, the NH2 of ARG is at
> 2.61A from ligand's oxygen atom (refer to attached image), since there is
> no electron density for hydrogens, how can i be sure if i am modelling
> ligand correctly?
>
> thanks
>
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