Dear members, We are planing to purify RNA only or/and RNA-protein complex on FPLC. The application of the purified materials is for crystallization. However, our concern is that our FPLC has been frequently used in bacterial expressing protein purification including purifying proteins from crude lysate, which is contaminated by RNase. Do you have experience of completely cleaning up the system before conducting the RNA work? Or it is impossible for 100% cleaning and we have to use a separate FPLC for this purpose?
Thank you. Best regards, Wim ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
