Hi 1. Lysate on fplc is messy :) which is why I always do the first step in batch.
2. A good scrubbing with NaOH, trypsin, and a strong chaotropic agent (in series) should take care of the contamination, however it may be easier to find e.g. an old HPLC never used for protein work in order to purify your material. If your RNA is single stranded, it will have a hard time surviving this ordeal. Of it is double stranded, should be no worries. Artem On Mon, Jun 21, 2021, 8:47 PM WENHE ZHONG <wenhezhong.xmu....@gmail.com> wrote: > Dear members, > > We are planing to purify RNA only or/and RNA-protein complex on FPLC. The > application of the purified materials is for crystallization. However, our > concern is that our FPLC has been frequently used in bacterial expressing > protein purification including purifying proteins from crude lysate, which > is contaminated by RNase. Do you have experience of completely cleaning up > the system before conducting the RNA work? Or it is impossible for 100% > cleaning and we have to use a separate FPLC for this purpose? > > Thank you. > > Best regards, > Wim > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
