Hello, could the His be partially acetylated? Best wishes Jon.C.
Sent from ProtonMail mobile -------- Original Message -------- On 16 Aug 2021, 14:52, Ana Ebrecht wrote: > Dear all, > > I am building the structure of a protein that was crystallized in 0.2 M > sodium malonate pH 5.0, 20% w/v polyethylene glycol 3,350. > During the refinement, we found what we think is a malonate molecule in the > active site, but it seems like is bound somehow to the histidine (this His is > the catalytic residue of the enzyme), almost like a covalent interaction. > Under other conditions of crystallization, the protein bound a sulfate and an > acetate in the site but did not show this type of interaction with the > histidine. > > We couldn't find anything that explains a reaction between the malonate and > the histidine. > Does anyone have experience with this reaction or have seen something similar > before? > > Thanks > Kind regards > Ana > > [malonate.jpg] > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
