Dear Chang, One need to set resolution cut off, to have a meaningful data without losing high resolution data and keeping data integrity. Some key quality indicators like I/Sigma I, CC 1/2 and Rpim etc., at outer most shell need to be considered. What was the CC 1/2 value in outer shell ?
Please refer to the below paper. How good are my data and what is the resolution Assessing and maximizing data quality in macromolecular crystallograph On Sat, 11 Sep 2021, 9:52 pm Tao-Hsin Chang, <[email protected]> wrote: > Hi Farhan, > > It looks like that your diffraction data has an anisotropic issue and it > leads to the issues of resolution limit, intensity, and completeness. Check > The STARANISO Server ( > https://staraniso.globalphasing.org/cgi-bin/staraniso.cgi). It may be > useful for your case. > > Best wishes, > Tao-Hsin > > On Sep 11, 2021, at 11:55 AM, Syed Farhan Ali <[email protected]> > wrote: > > Dear All, > > I have query regarding one of my dataset. I am running aimless by keeping > highest resolution 1.62 A and getting I/SigI = 2 but data completeness is > around 22 in outermost shell. And if I am increasing the resolution cutoff > up to 1.8 A then I/SigI is 6.2 and completeness is 82.4. > I have attached the screenshot of the result. > What should be the criteria to set the resolution limit? Should I stick > to I/SigI or I have to consider about the completeness of data. > And if completeness is also a guiding factor than how much minimum > completeness I can keep in the higher resolution shell. > > > > > > Regards, > Farhan > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <Screenshot 2021-09-11 at 8.43.25 PM.png><screenshot1.6.tiff> > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
