Along with the paper mentioned by Rajiv, you could look at this paper as well which discusses a major shift in the understanding of data quality from I/sig(I) based to CC1/2 based indicators.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684713/ Hope this helps. All the best. Best, Tushar. On Sat, 11 Sep 2021, 09:36 Rajiv gandhi.s, <[email protected]> wrote: > Dear Chang, > One need to set resolution cut off, to have a meaningful data without > losing high resolution data and keeping data integrity. Some key quality > indicators like I/Sigma I, CC 1/2 and Rpim etc., at outer most shell need > to be considered. What was the CC 1/2 value in outer shell ? > > Please refer to the below paper. > How good are my data and what is the resolution > Assessing and maximizing data quality in macromolecular crystallograph > > On Sat, 11 Sep 2021, 9:52 pm Tao-Hsin Chang, <[email protected]> > wrote: > >> Hi Farhan, >> >> It looks like that your diffraction data has an anisotropic issue and it >> leads to the issues of resolution limit, intensity, and completeness. Check >> The STARANISO Server ( >> https://staraniso.globalphasing.org/cgi-bin/staraniso.cgi). It may be >> useful for your case. >> >> Best wishes, >> Tao-Hsin >> >> On Sep 11, 2021, at 11:55 AM, Syed Farhan Ali <[email protected]> >> wrote: >> >> Dear All, >> >> I have query regarding one of my dataset. I am running aimless by keeping >> highest resolution 1.62 A and getting I/SigI = 2 but data completeness is >> around 22 in outermost shell. And if I am increasing the resolution cutoff >> up to 1.8 A then I/SigI is 6.2 and completeness is 82.4. >> I have attached the screenshot of the result. >> What should be the criteria to set the resolution limit? Should I stick >> to I/SigI or I have to consider about the completeness of data. >> And if completeness is also a guiding factor than how much minimum >> completeness I can keep in the higher resolution shell. >> >> >> >> >> >> Regards, >> Farhan >> >> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> <Screenshot 2021-09-11 at 8.43.25 PM.png><screenshot1.6.tiff> >> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
