Christine - can you attach the relevant log files! Eleanor

On Fri, 17 Dec 2021 at 22:59, Ethan A Merritt <[email protected]> wrote:

> On Friday, 17 December 2021 14:04:17 PST Christine Gee wrote:
> > Hi,
> >
> > I recently came across this strange issue. I was using aimless in CCP4 to
> > scale my data and apply an Rfree from a reference .mtz
> >
> > Neither the reference or the file I was working with had higher
> resolution
> > than 1.5
> > However aimless wrote out h, k, l and Rfree to 0.4. I did not click the
> > button to extend resolution by the way.
> >
> > No big deal right? Well Coot then assumed the resolution was 0.4 and
> wrote
> > out maps to super high resolution and everything took forever to render,
> > making coot basically unuseable.
>
> Coot->File
>         ->Open mtz
>                 select mtz file by name
>                 ->Expert Mode
>                         ->Use Resolution Limits
>                         set high resolution limit to 1.5
>
> However, I would worry that the spurious resolution is symptom of
> a more pervasive problem.
>
>         Ethan
>
>
> >
> > I could not find any global or local parameter in coot that seemed to
> > remedy this.
> >
> > Did anyone come across anything similar? What is the solution besides
> > editing the resolution of the .mtz file output from aimless and what
> > program woud you recommend for this?
> >
> > My simple solution for now was to use an .mtz file output from aimless
> that
> > a reference Rfree was not applied to.
> >
> > Regards
> > Christine
> >
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>
> --
> Ethan A Merritt
> Biomolecular Structure Center,  K-428 Health Sciences Bldg
> MS 357742,   University of Washington, Seattle 98195-7742
>
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