Dear Dom,

On Fri, Jan 14, 2022 at 10:56:32PM +0000, Dom Bellini wrote:
> Thanks for your inputs. I wonder why the PDB doesn’t allow to submit
> two separate mtz files,

Ideally, these would actually be mmCIF files since MTZ files (1) can not hold
all meta data a PDBx/mmCIF compatible file could potentially carry and (2) it
will require a conversion after upload. Of course, if your processing/refinement
software doesn't produce mmCIF files you are limited to that format and the
online tool Marcin mentioned is probably the best way to go.

> one from data processing and one from refinement with map
> coefficients.

If your processing and refinement package produces mmCIF files directly, it
would be best to take them as-is into deposition.

As an example, the combination autoPROC [1] and BUSTER [2] allows for this [3]:
taking the mmCIF file from data processing (including scaled merged and unmerged
data with enriched meta data and full data quality metrics) plus the mmCIF file
from refinement (including map coefficients in various forms) and combining them
via the aB_deposition_combine command (which takes care of alternative indexing,
test-set flags etc, since it might not be just a case of concatenating ASCII
mmCIF files together). This should work in creating a full reflection mmCIF with
all relevant types of reflection data.

There are probably similar systems in place to achieve something very similar in
other packages.

Cheers

Clemens

[1] https://www.globalphasing.com/autoproc
[2] https://www.globalphasing.com/buster
[3] https://www.globalphasing.com/buster/wiki/index.cgi?DepositionMmCif

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