Dear Guenter,
Would the use of vials inside combi-pucks
(https://www.mitegen.com/product/combipuck-system/) and some
arrangements with your local contact at the other end, perhaps help with
using propane remotely?
BW,
D
On 26/01/2022 16:53, Guenter Fritz wrote:
Dear all,
we have some delicate crystals which might benefit from freezing in
propane. In former times (when I was still travelling to the
beamlines) we waited until the propane was solid in the vial and then
let the propane thaw in the cryo stream at the beamline.
But how can we do this in these days with unipucks and no manual
mounting?
I was thinking about freezing in ethane and then transfer the loops to
liquid nitrogen, similarly we handle grids for cryo EM. Does somebody
has tried that? Any experience, tips & tricks would be very welcome!
Thanks in advance and best regards,
Guenter
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Dom Bellini, Xray Crystallography Facility (1S205)
MRC Laboratory of Molecular Biology
Francis Crick Avenue
Cambridge Biomedical Campus
Cambridge CB2 0QH
Phone 01223 267839
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