Hi Monika, I would process the data using the point group information ignoring any possible screw axes, e.g. in space group P222, where the true space group might be P212121.
The next step depends on how you plan to solve the structure. You mention that there is no cryo structure, do you mean cryoEM structure, or that you cannot freeze the crystals? If there is any search model available (crystal, cryoEM, alphaFold), I would run molecular replacement, using all possible space groups for your point group. This should resolve the correct space group. Good luck! Herman Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> Im Auftrag von Monika Bjelcic Gesendet: Freitag, 22. Juli 2022 11:51 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Determining space group Hi, I’m hoping someone can help me how to determine a space group from my collection. I did serial crystallography on a crystal that doesn’t have a cryo structure. I was able to determine the Point group but for the next step I’m stuck. Kind regards, Monika Bjelcic PhD student at BioMAX [cid:image001.jpg@01D89DC5.033B5D90] MAX IV Laboratory Lund University Postal address: P.O. Box 118, SE-221 00 Lund, Sweden Visiting address: Fotongatan 2, 224 84 Lund, Sweden Mobile: +46-761357994 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
