Most molecular replacement programs allow you to search in all possible space groups consistent with a given point group. Eleanor
On Fri, 22 Jul 2022 at 11:18, Schreuder, Herman /DE < herman.schreu...@sanofi.com> wrote: > Hi Monika, > > > > I would process the data using the point group information ignoring any > possible screw axes, e.g. in space group P222, where the true space group > might be P212121. > > > > The next step depends on how you plan to solve the structure. You mention > that there is no cryo structure, do you mean cryoEM structure, or that you > cannot freeze the crystals? If there is any search model available > (crystal, cryoEM, alphaFold), I would run molecular replacement, using all > possible space groups for your point group. This should resolve the correct > space group. > > > > Good luck! > > Herman > > > > *Von:* CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> *Im Auftrag von *Monika > Bjelcic > *Gesendet:* Freitag, 22. Juli 2022 11:51 > *An:* CCP4BB@JISCMAIL.AC.UK > *Betreff:* [ccp4bb] Determining space group > > > > Hi, > > > > I’m hoping someone can help me how to determine a space group from my > collection. > > I did serial crystallography on a crystal that doesn’t have a cryo > structure. I was able to determine the Point group but for the next step > I’m stuck. > > > > Kind regards*,* > > *Monika Bjelcic* > > PhD student at BioMAX > > *MAX IV Laboratory* > > Lund University > > Postal address: P.O. Box 118, SE-221 00 Lund, Sweden > > Visiting address: Fotongatan 2, 224 84 Lund, Sweden > > Mobile: +46-761357994 > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
