I second glycerol.
Best regards, tom

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From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of kotping 
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Sent: Tuesday, December 6, 2022 6:02:00 PM
To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Interesting Density

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Glycerol

-----Original message-----
From:John Smith<proteinstructur...@gmail.com>
To:CCP4BB<CCP4BB@JISCMAIL.AC.UK>
Date: Tue, 06 Dec 2022 14:05:35
Subject: [ccp4bb] Interesting Density
Dear all!

I noticed some interesting density at the surface of my protein.
It looks like a distorted T - when I change the Fo-Fc to 5.5 sigma one arm is 
still visible, one weakly and the other one disappeared.  Crystallisation 
condition has Hepes, NaCl, NaSCN, TCEP and PEG3350. Any ideas what this could 
be?

I appreciate any help you can provide. Best John

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Tzu-Ping  Ko,  PhD
Staff  Research  Assistant
Institute  of  Biological  Chemistry
Academia  Sinica,  Taipei,  Taiwan
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