Hi Bashir, It does look like you have translational pseudo-symmetry. If the translation vector happens to be body-centering, it could affect your intensity statistics so that the R-values would never go down as half of your reflections follow the distribution for centric reflections. I had this a long time ago; you might want to check https://doi.org/10.1107/S0907444906031519.
HTH, Esko Sent from my iPhone On 27 Jun 2023, at 19:03, Muhammad Bashir Khan <[email protected]> wrote: Hello All; I have x-rays data of about 100 kDa protein at 3.3A. It gives MR statistics as LLG 150 and TFZ 7 but when I switched off the tNCS I got an LLG around 2500 and TFZ 45, but the R and Rfree are stuck at 39 and 43. The space group is 4. Any suggestion in this regard will be highly appreciated. Patterson's analysis is attached. Thank you all Bashir -- ------------------------------------------------------ Muhammad Bashir Khan, Ph.D. Research Associate Department of Biochemistry Medical Science Bldg. Lab 3-27 University of Alberta Edmonton AB, T6G 2H7 Phone: 780-492-4577- e-mail: [email protected]<mailto:[email protected]> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 <Patterson_peak.jpg> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
