WELL - with such high LLG and translation NCSthe high R factors are a bit surprising.. The Patterson info does not give the relative height of the origin peak to the off origin one.. Questions which I would check. Look at the data processing carefully. Are there bad batches? Could the space group be wrong? Is the Wilson plot linear? Any twinning? etc..
How many molecules do you expect in the asymmetric unit? And so on. I can look at your log files if you like and try to see if they help. Eleanor On Tue, 27 Jun 2023 at 18:03, Muhammad Bashir Khan <[email protected]> wrote: > Hello All; > > I have x-rays data of about 100 kDa protein at 3.3A. It gives MR > statistics as LLG 150 and TFZ 7 but when I switched off the tNCS I got an > LLG around 2500 and TFZ 45, but the R and Rfree are stuck at 39 and 43. The > space group is 4. Any suggestion in this regard will be highly appreciated. > Patterson's analysis is attached. > > Thank you all > > Bashir > > -- > ------------------------------------------------------ > Muhammad Bashir Khan, Ph.D. > Research Associate > Department of Biochemistry > Medical Science Bldg. > Lab 3-27 > University of Alberta > Edmonton AB, T6G 2H7 > > Phone: 780-492-4577- > e-mail: [email protected] <[email protected]> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
