Dear kavya
There is precedent for this. Check the reference below. This protein was
crystalized from solution at a concentration of 150 mg/ml. Keep
concentrating, I guess !
Cell. Volume 98, Issue 4
<https://www.sciencedirect.com/journal/cell/vol/98/issue/4>, 20 August
1999, Pages 537-546
https://doi.org/10.1016/S0092-8674(00)81981-9
Best regards
Josan
_________________________________________________________
Jose A. Marquez, Senior Scientist
Head of the Crystallization Facility
European Molecular Biology Laboratory, Grenoble.
Delivery address: EMBL, 71, Avenue des Martyrs
38000 Grenoble, France
Postal address: EMBL, 71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.org/
_________________________________________________________
On 2/5/2024 11:27 AM, kavyashreem wrote:
Dear All,
Has anyone worked on a protein which is highly soluble even at 80mg/ml?
We have one such candidate, which does not precipitate even at 80mg/ml
instead forms phase separated globules in crystallization plate, which
eventually hardens over a period of 1 to 1.5 months (which is
florescent under UV microscope.)
We tried screening at different pH, but failed to get any hits.
Since we got few conditions in which the phase separated globules
solidified, we focused on them and expanded with 120mg/ml protein,
still there were not visible precipitates except for the phase
separation. This has been a challenging target so far. We have tried
with different constructs, which unfortunately are not soluble!
Does POMs help in such cases? Or do you have any other suggestion.
Thank you
Regards
Kavya
*CONFIDENTIAL: This email and any files transmitted with it are
confidential and intended solely for the use of the individual or
group to whom they are addressed. If you have received this email in
error, please notify the sender immediately and delete this e-mail
from your system. It is NOT AUTHORISED to copy, forward, or in any way
reveal the contents of this message to anyone.*
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1>
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list
hosted by www.jiscmail.ac.uk, terms & conditions are available at
https://www.jiscmail.ac.uk/policyandsecurity/
