Hi,
I have a small protein that is hugely soluble too.
We could crystallize it in 3.5M ammonium sulfate with the help of a
ligand that apparently triggered the crystallization.
Try changing the temperature too.
Good luck,
GIA
Le 05/02/2024 à 11:27, kavyashreem a écrit :
Dear All,
Has anyone worked on a protein which is highly soluble even at 80mg/ml?
We have one such candidate, which does not precipitate even at 80mg/ml
instead forms phase separated globules in crystallization plate, which
eventually hardens over a period of 1 to 1.5 months (which is
florescent under UV microscope.)
We tried screening at different pH, but failed to get any hits.
Since we got few conditions in which the phase separated globules
solidified, we focused on them and expanded with 120mg/ml protein,
still there were not visible precipitates except for the phase
separation. This has been a challenging target so far. We have tried
with different constructs, which unfortunately are not soluble!
Does POMs help in such cases? Or do you have any other suggestion.
Thank you
Regards
Kavya
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