Hi everyone, Sorry for the off-topic question, but I thought I may get some advice/pointers about the design of a stable alpha-helical peptide. I would like to obtain a peptide mimicking a 13-residue alpha-helix that was identified in a protein to mediate a protein-protein interaction, which is completely abolished by three spatially adjacent point mutations in the helix of the protein. I designed a peptide with a 1-residue overhang on each side and a 6-carboxy-fluorescein moiety on one end, but the peptide did not bind to the interaction partner probed by fluorescence polarization. The peptide easily dissolves in water, so solubility does not seem to be an issue. Any suggestions or pointers to relevant literature would be greatly appreciated.
Cheers! Erik ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
