Hello all, I am currently trying ccp4 cloud for structure solution of a 4 domain protein, 543 amino acids monomeric protein. I split the aplhaphold model into 4 domains, and prepared a single chain ensemble with the 4 domains . I see a good fit for two of the C-terminal large domain that appear to be in a correct/similar position compared to other known structures of homologs and the alphafold model. the 3rd domain from the c-terminus also fits well in the map but is in a different part of the unit cell, and nowhere near the other two C-terminal domains that also fit the map well. Finally, the most N-terminal domain does not fit the map at all. What is the best way to move forward with this mr solution from phaser in ccp4 Cloud? I am looking to move forward with the seemingly correct placed coordinates to try to get a better solution. Also I do not necessarily require to use exclusively ccp4 cloud I just appreciate its cloud based speed.
Thank! Marco ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
