Hi Marco, I would try MR with the N-terminal domain only separately, or the domains that fit well in one single chain, and the N-terminal domain other chain and try to find these two in the map with MR.
Good luck, Burak Marco Bravo <[email protected]>, 10 Şub 2025 Pzt, 06:17 tarihinde şunu yazdı: > Hello all, > I am currently trying ccp4 cloud for structure solution of a 4 domain > protein, 543 amino acids monomeric protein. I split the aplhaphold model > into 4 domains, and prepared a single chain ensemble with the 4 domains . I > see a good fit for two of the C-terminal large domain that appear to be in > a correct/similar position compared to other known structures of homologs > and the alphafold model. the 3rd domain from the c-terminus also fits well > in the map but is in a different part of the unit cell, and nowhere near > the other two C-terminal domains that also fit the map well. Finally, the > most N-terminal domain does not fit the map at all. What is the best way to > move forward with this mr solution from phaser in ccp4 Cloud? I am looking > to move forward with the seemingly correct placed coordinates to try to get > a better solution. Also I do not necessarily require to use exclusively > ccp4 cloud I just appreciate its cloud based speed. > > Thank! > > Marco > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
