Dear Michael, It appears to me like a PEG molecule with two alternative conformations at its terminus and a bound water molecule?
Nice maps! Tom Huxford. ============== Tom Huxford Structural Biochemistry Laboratory Department of Chemistry & Biochemistry San Diego State University (619) 594-1606 > On Feb 27, 2025, at 11:57 AM, Kennedy, Michael <[email protected]> > wrote: > > Hello All, > > We recently solved the structure of a protein at 1.8A. There is a large > unknown density (see attached) at the interface between two protein > molecules. > > The crystallization buffer was 0.2 M Li Sulfate, 0.1 M Tris,25% PEG 4000, 10% > glycerol. > The protein purification buffer was 250 mM NaCl, 20 mM Tris, 10% glycerol, > 300 mM imidazole. > > Does anyone recognize this ligand or have an idea about what it might be? > > thanks > > Michael A. Kennedy, PhD > > Eminent Scholar and Professor > Department of Chemistry and Biochemistry > 106 Hughes Laboratory > Miami University > 651 East High Street > Oxford, OH 45056 > > phone: 513-529-8267 > fax: 513-529-5715 > email: [email protected] <mailto:[email protected]> > webpage: http://chemistry.muohio.edu/kennedy/ > google scholars citations: > https://scholar.google.com/citations?user=7rWzfjkAAAAJ > NCBI My Bibliography > https://www.ncbi.nlm.nih.gov/myncbi/1L3_kwfzxCU/bibliography/public/ > Lab Twitter : @Kennedy_Lab > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > <Screen Shot 2025-02-27 at 1.06.57 PM.png><Screen Shot 2025-02-27 at 1.41.29 > PM.png> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
