Hi Michael,
It looks like PEG to me too. Have you used a smaller PEG as cryoprotectant.
Such as PEG 200 or PEG400? Also, as an off topic thing, why are you refining
hydrogens at this resolution?
Best wishes
______________________________________________________
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +44 07861 273773
"A sorte acompanha uma mente bem treinada"
________________________________________________
________________________________
De: CCP4 bulletin board <[email protected]> em nome de Kennedy, Michael
<[email protected]>
Enviado: quinta-feira, 27 de fevereiro de 2025 19:57
Para: [email protected] <[email protected]>
Assunto: [ccp4bb] unknown density
Hello All,
We recently solved the structure of a protein at 1.8A. There is a large unknown
density (see attached) at the interface between two protein molecules.
The crystallization buffer was 0.2 M Li Sulfate, 0.1 M Tris,25% PEG 4000, 10%
glycerol.
The protein purification buffer was 250 mM NaCl, 20 mM Tris, 10% glycerol, 300
mM imidazole.
Does anyone recognize this ligand or have an idea about what it might be?
thanks
Michael A. Kennedy, PhD
Eminent Scholar and Professor
Department of Chemistry and Biochemistry
106 Hughes Laboratory
Miami University
651 East High Street
Oxford, OH 45056
phone: 513-529-8267
fax: 513-529-5715
email: [email protected]<mailto:[email protected]>
webpage: http://chemistry.muohio.edu/kennedy/
google scholars citations:
https://scholar.google.com/citations?user=7rWzfjkAAAAJ
NCBI My Bibliography
https://www.ncbi.nlm.nih.gov/myncbi/1L3_kwfzxCU/bibliography/public/
Lab Twitter : @Kennedy_Lab
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