Dear Mohit,
In addition to all the useful suggestions already mentioned, you might
be interested in testing crystallophore, a nucleating agent, which acts
as a molecular glue to provide new crystallization conditions or improve
existing ones.
See https://doi.org/10.1039/C7SC00758B and
https://doi.org/10.1107/S1600576719006381
The crystallophore can be purchased from Mitegen/Molecular
Dimensions/Polyvalan.
Best of luck,
Eric.
Le 30/06/2025 à 09:17, Mohit Bhardwaj a écrit :
Dear members,
I am currently working on the crystallization of the main protease
(3CLpro) of SARS-CoV-2, focusing on both the wild-type and several
point mutants.
Initial crystallization trials were conducted using commercial
screens, including PACT, JCSG+, and INDEX. These trials yielded
crystals of various morphologies—predominantly needles, thin sheets,
and flower-like clusters. To improve crystal quality, I subsequently
performed optimization using the hanging-drop vapor diffusion method
by systematically varying PEG concentration, salt
types/concentrations, and pH conditions. Despite these efforts, the
crystals have not shown significant improvement in morphology or
diffraction quality.
I have also attempted microseeding and macroseeding approaches during
optimization, but the results remain largely the same. I would greatly
appreciate any suggestions or experiences from the community regarding
strategies that could help improve crystal quality in such systems,
particularly with challenging morphologies like needle or sheet-like
forms.
Thank you in advance for your insights.
Mohit Bhardwaj
PhD Scholar
Kusuma School of Biological Sciences
IIT Delhi, Hauz Khas
New Delhi- 110016
Mobile No. : +91-8895172936, 8700227218
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1>
--
****************************************************************
Eric Girard
Extremophiles and Large Macromolecular Assemblies (ELMA) group
&
Synchrotron group
Institut de Biologie Structurale
UMR 5075 CEA-CNRS-UGA-PSB
71 avenue des Martyrs
CS 10090
38044 Grenoble Cedex 9
Phone: +33 (0)4 57 42 86 45
Fax: +33 (0)4 76 50 18 90
Web site:http://www.ibs.fr/
*****************************************************************
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list
hosted by www.jiscmail.ac.uk, terms & conditions are available at
https://www.jiscmail.ac.uk/policyandsecurity/
