Dear All, the smallest centrifugal protein concentrators I know take sample volumes of 0.5 ml, like these: https://www.sigmaaldrich.com/ES/es/product/mm/ufc5010 (but other brands are also available).
In theory you should be able to get to less than 40. In practice it is difficult. A problem is that, with a total protein amount of below 0.5 mg, a significant amount of it will stick to the plastic of the tubes and the filter, so it just becomes impossible to get very small volumes. I was wondering if there are other solutions available? Or efforts to miniaturise this? (apart from lyophilisation, which is not suitable for many proteins). Best wishes, Mark Mark van Raaij Dpto de Estructura de Macromoleculas, lab B5B Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. +34 91 585 4616 (internal 432092) ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
