Hello all, I have a 4.0 A crystal structure of an enzyme-ribosome complex, and because of the poor map quality, I have enhanced the map quality by using the feature-enhanced map (FEM) for crystal structures and the autosharpen function available for cryo-EM structures in PHENIX.
I would like to know if I need to show the unsharpened map (the normal 2Fo-Fc map) along with the sharpened maps for the region of interest? In some instances, the unsharpened map does not show density for certain residues, therefore is it OK to solely rely on the FEM? Also, is it correct to use the autosharpen function that is available for cryo-EM structures but not for xtals? Best, Suparno Nandi, Ph.D. Dept. of Biochemistry Emory University Atlanta, GA, USA ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
