Hi Suparno, Show the original standard map and the improved map side by side, and describe the steps you used to arrive at the more interpretable map in a reproducible manner. To ensure reproducibility, write this up and ask your colleague to reproduce it based on your description. If they succeed, you're golden, and then it will be up to the referees (and the rest of the world) to challenge your conclusions.
Good luck, Pavel On Tue, Jan 27, 2026 at 10:07 AM Suparno Nandi < [email protected]> wrote: > Hello all, > > I have a 4.0 A crystal structure of an enzyme-ribosome complex, and > because of the poor map quality, I have enhanced the map quality by using > the feature-enhanced map (FEM) for crystal structures and the autosharpen > function available for cryo-EM structures in PHENIX. > > I would like to know if I need to show the unsharpened map (the normal > 2Fo-Fc map) along with the sharpened maps for the region of interest? In > some instances, the unsharpened map does not show density for certain > residues, therefore is it OK to solely rely on the FEM? Also, is it correct > to use the autosharpen function that is available for cryo-EM structures > but not for xtals? > > Best, > > Suparno Nandi, Ph.D. > Dept. of Biochemistry > Emory University > Atlanta, GA, USA > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
