Hello Everyone, I am seeking advice on selecting the appropriate cell lines for the expression of a secreted protein that is over 180 kDa in size. I am particularly interested in conducting expression experiments using mammalian cells. I have come across several research papers that mention the use of HEK-derived cell lines, such as HEK293 GnTI-, Expi293, and FreeStyle 293F.
I would greatly appreciate your insights on how to choose the best cell line to achieve a high protein expression yield, as this specific protein has shown suboptimal expression in other systems. Thank you for your help. I look forward to your response. Regards, -- Anamika Singh, Ph.D. Research Associate ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
