I have used all three lines. I use the cells adapted to the same media for comparison for reduced costs (ie not the kit or recommended media they are normally sold with). Doing it this way the difference is not exceptional, so we just use normal 293E cells adapted to suspension. If you are using the supplied kit reagents and media, Expi293 does work exceptionally.
There is also ExpiPro293 now. We typically find secreted proteins are better produced in CHO (ie ExpiCHO), and that is also the industry standard for most biologics. Your construct, signal peptide and codons also make a big difference. Best Kelvin -- Kelvin Lau https://people.epfl.ch/kelvin.lau Schedule a 30 minute meeting<https://outlook.office.com/bookwithme/user/[email protected]?anonymous&ismsaljsauthenabled&ep=plink> Scientist - Protein production and structure core facility – PTPSP President – EPFL Scientific Staff Association – ELSA EPFL SV PTECH PTPSP AI 2146 (Bâtiment AI) Station 19 CH-1015 Lausanne Switzerland Email: [email protected]<mailto:[email protected]> Phone: +41 21 69 30267<tel:+41%2021%2069%C2%A030267> If unreachable: +41 21 69 34494<tel:+41%2021%2069%C2%A030267> On 11.02.2026, 16:07, "CCP4 bulletin board" <[email protected]> wrote: Hello Everyone, I am seeking advice on selecting the appropriate cell lines for the expression of a secreted protein that is over 180 kDa in size. I am particularly interested in conducting expression experiments using mammalian cells. I have come across several research papers that mention the use of HEK-derived cell lines, such as HEK293 GnTI-, Expi293, and FreeStyle 293F. I would greatly appreciate your insights on how to choose the best cell line to achieve a high protein expression yield, as this specific protein has shown suboptimal expression in other systems. Thank you for your help. I look forward to your response. Regards, -- Anamika Singh, Ph.D. Research Associate ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
