Dear all, I am currently processing a SAD dataset collected from a Se-Met derivative. According to the matthews_coef calculation in CCP4, there should be 20 heavy atom sites in the asymmetric unit. However, due to the very weak anomalous signal, the occupancy refinement in SHELX shows only one site with an occupancy above 1.0, one site around 0.4, and the remaining 15 sites all converging to approximately 0.3. Given this situation, I would be very grateful for any suggestions on how to obtain reliable initial phases. Are there any specific strategies, software settings, or alternative approaches (e.g., density modification or partial structure refinement) that you would recommend to make the most of this weak signal? Thank you in advance for your time and insights. Best regards, Kai Zhang
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