Interesting little study, Leanne.  Thanks for sharing.  Myra

>From: Leanne Bertani <[EMAIL PROTECTED]>
>Reply-To: The Cavalier King Charles Spaniel List
><[EMAIL PROTECTED]>
>To: [EMAIL PROTECTED]
>Subject: [CKCS-L] frequency of sperm collection in dogs with abnormal sperm
>Date: Sun, 10 Feb 2002 16:28:43 -0500
>
>J Vet Med Sci 1998 May;60(5):607-14
>
>
>Changes in semen quality and in vitro sperm capacitation during various
>frequencies of semen collection in dogs with both asthenozoospermia and
>teratozoospermia.
>
>Kawakami E, Hori T, Tsutsui T.
>
>Department of Reproduction, Nippon Veterinary and Animal Science
>University,
>Tokyo, Japan.
>
>Eight male dogs with both asthenozoospermia and teratozoospermia were used
>in this study. In experiment 1, semen was collected 10 times at intervals
>of
>48 hr, 24 hr and 12 hr in 4 of the 8 dogs, and the semen quality was
>evaluated. In experiment 2, semen was collected 5 times at 24-hr intervals
>in the other 4 dogs. The spermatozoa collected on day 1 and day 5 were
>incubated for 4-6 hr in Canine Capacitation Medium, and the percentages of
>hyperactivated sperm (%HA), acrosome-reacted sperm (%AR), and the zona
>pellucida-binding sperm count (ZP sperm count) were assessed. The results
>of
>experiment 1 showed that the percentage of motile sperm increased and the
>percentage of abnormal sperm decreased markedly as the intervals between
>semen collections became shorter. When semen was collected at 12-hr
>intervals, the percentage of motile sperm increased from about 65%, the
>value before frequent collection was started, to about 80%, and the
>percentage of sperm with abnormal tails decreased from 30% to 15%. In
>experiment 2, the percentages of HA and AR, and the ZP sperm count in
>specimens collected on day 5 were higher than those in specimens collected
>on day 1, and the differences in % HA and in ZP sperm count were
>significant
>(P<0.05). The results demonstrated that sperm motility, abnormality, and
>potential fertility in dogs with asthenozoospermia and teratozoospermia can
>be temporarily improved by frequent semen collection.
>
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Myra Savant  Gardengate Cavaliers


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