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OBJECTIVE: To investigate the effect of specific small interfering RNA
(siRNA) targeting against bcr-abl chimeric gene on the biological traits of
chronic myelogenous leukemia (CML) cells. METHODS: CML cells of the line K561
transcribing a type of b3: a2 mRNA of bcr-abl chimeric gene were cultured. A
21nt siRNA targeting against the chimeric location of the b3: a2 mRNA of bcr-abl
chimeric gene was designed, synthesized, and transfected into the K562 cells as
RNA interference group. Another K562 cells were transfected with fluorescein
enzyme gene specific siRNA as indifferent controls, or with lipid alone as blank
vector controls.
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