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Chronic myeloid leukemia (CML) is caused by Bcr-Abl, an activated tyrosine
kinase. The amounts of Bcr-Abl mRNA and protein in cells from patients in blast
crisis (BC) are higher than in those chronic phase (CP), indicating that their
_expression_ rises with disease progression. In order to study this phenomenon on
cells with the same genetic background, we transfected the 32D cell line with
the BCR-ABL transgene and selected clones with graded _expression_ of Bcr-Abl
within the range found in cells from CP to BC.
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