Thanks, I thought it sounded good but my platelets are still a little high; 509 on this test; WBC 7.9. I can't understand why on some test there are no abnormal cells and on other test there are. It's confusing. Thanks again, Jeanie<3 In a message dated 2/4/2008 11:00:20 A.M. Pacific Standard Time, [EMAIL PROTECTED] writes:
No abnormal cells is good. Polyclonal is good. It means a diverse population of lymphocytes instead of an expansion of a single clone (leukemia). Most Lymphocytic leukemias are B-cell in origin Granulocytic cells are your Neutrophils, Eosinophils, and Basophils. No problems there. Immature granulocytic (aka myeloid) cells which can be indicative of other leukemias can be detected by flow cytometry. In cytometry, your cells are divided among a bunch of tubes and test reagents are added that have fluroescent dyes attached. Each tube is labeled with the names of the tests and about 6 can be added to each tube. Then the cells are sucked up into the cytometer and passed thru a laser beam. Cells are measured by the way they bend the laser light and also what type of flurescence light they emit. Gating is just the way the results are sorted out on the computer. Each cell that is analyzed on the cytometer (it can read about 10,000 a second) are stored in a computer file and its one file per test tube. The technician then goes back in the computer and puts gates (boxes) around the cell populations and asks the computer to show the results just for the cells in the gate. On a cytometer, the cell populations form kind of an arc on the screen with the lymphocytes in the lower left, the monocytes mid right, and the granulocytes in the upper middle to upper right. The idea with gating is just to look at what is of interest depending on the test reagents that were put into that tube. If the tube had no lympocyte-specific reagents in it, then there is no need to even count the lymphocytes in the analysis. A common test for clonal B-cell expansion is to test CD5 and CD19 (CD20) in the same tube. CD5 is usually only on T-cells. CD19 is on all B-cells and CD20 is only on mature B-cells. Every person with Chronic Lymphocytic Leukemia will have leukemia cells that exhibit both CD5 and CD19 (or CD20) on the same cell. That is not normal. If each reagent was done separately, one might be able to infer that something was wrong since the % of B-cells in the blood was way too high but this simple combination confirms the diagnosis immediately. Cytometry can be quite useful as a screening tool of the blood. This report looks great. On Feb 4, 1:01 pm, [EMAIL PROTECTED] wrote: > Hi all, > Here is the results of the FLOW CYTOMETRY REPORT done on my Peripheral blood. > IMMUNOPHENOTYPE REPORT > SPECIMEN : Peripheral blood > > INTERPRETATION > There is no immunophenotypic evidence of an abnormal population of cells. > IMMUNOPHENOTYPE (of relevant population); Polyclonal B-lymphocytes, normal > T-lymphocytes and granulocytic cells are identified. > ANALYTICAL GATING: > Gating was performed on the granulocytic, blast, monocytic and lymephocytic > region > MORPHOLOGY: > Review of the peripheral blood smear demonstrates no evidence of circulating > abnormal cells > CLINICAL DATA: > The patient is a 69 year old female with history of thrombocytosis and > leukocytosis > Can someone explain this to me. > Thanks, > Jeanie<3 **************Biggest Grammy Award surprises of all time on AOL Music. (http://music.aol.com/grammys/pictures/never-won-a-grammy?NCID=aolcmp003000000025 48) --~--~---------~--~----~------------~-------~--~----~ [CMLHope] A support group of http://cmlhope.com ------------------------------------------------- You received this message because you are subscribed to the Google Groups "CMLHope" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [EMAIL PROTECTED] For more options, visit this group at http://groups.google.com/group/CMLHope -~----------~----~----~----~------~----~------~--~---
