Dear Anil, Dabur labs is a good lab and trustworthy. If you look at the note at the bottom of the RT-PCR report, you will see that they can only quantify transcripts corresponding to the p190 and p210 proteins. Although rare, some CML patients have unusual BCR-ABL breakpoints and if they do, most PCR labs are not able to give a measurement of the disease by PCR. For example, a few patients will have a p230 BCR-ABL protein and if they do, the Dabur PCR will not be able to measure the disease load. The PCR labs use primers for the major and minor breakpoints only and if Aditya has a rare breakpoint, his PCR at this lab will always be zero.
I have met 2 other patients who had rare BCR-ABL breakpoints and their doctors followed their disease by FISH and bone marrow cytogenetics testing. Since both cytogenetics (30%Ph+) and FISH (12%Ph +) show Aditya is still not in complete cytogenetic remission (CCR), most doctors would increase the dose to see if the Ph percentage goes down. So, I think in Aditya's case, monitoring by FISH and BMA cytogenetics will give a measure of his disease load. I wish Aditya good luck on the increased dose. Please do not hesitate to email me if I can help further. Warm Regards, Anjana wife of Roy d/x Jan 2002 400mg Glivec PCR 3.9log On Mar 26, 8:58 pm, "anil nagpal" <[EMAIL PROTECTED]> wrote: > Dear Anjana, > > I am Anil Nagpal F/o Aditya M/ 10 yrs d/x March 2005 , on Gleevec 300 for > one yr and then on 400mg from April 06 onwards. > BMB test as on 15 Feb 07 shows 20% PH+ve but RT -PCR test at the same time > shows 0% . RT -PCR and FISH report of June 06 is enclosed the result was > same in Feb 07 also 0%. > > BMB was done again on 10th March 08 and shows 30% Ph+ve. > > Doctor has advised to increase the dose to 500 mg > > I am sure that if I will get the RT PCR test done now it will again show 0%. > > Pls give your views on this and on which test I should trust more. > > regards > > Anil Nagpal > - --~--~---------~--~----~------------~-------~--~----~ [CMLHope] A support group of http://cmlhope.com ------------------------------------------------- You received this message because you are subscribed to the Google Groups "CMLHope" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [EMAIL PROTECTED] For more options, visit this group at http://groups.google.com/group/CMLHope -~----------~----~----~----~------~----~------~--~---
