Dear All, I am building and refining a protein-DNA complex at 3 Ang resolution and having a bit of trouble with side-chains that interact with DNA. In particular Arg side-chains that H-bond to the phosphate backbone tend to get dragged into the phosphate density when I do real space refinement. I have tried playing about with the weight matrix in the R/RC menu, but this doesn't seem to help much. I am running version 0.6 for Mac (Miramar revision 2540).
Any help much appreciated. Thanks Dave ------------------------------- Dr. David M. Lawson Biological Chemistry Dept., John Innes Centre, Norwich, NR4 7UH, UK. Tel: +44-(0)1603-450725 Fax: +44-(0)1603-450018 Email: [email protected]<mailto:[email protected]> Web: http://www.jic.bbsrc.ac.uk/staff/david-lawson/index.htm
