On 04/20/2011 01:57 PM, david lawson (JIC) wrote:
Dear All,
I am building and refining a protein-DNA complex at 3 Ang resolution
and having a bit of trouble with side-chains that interact with DNA.
In particular Arg side-chains that H-bond to the phosphate backbone
tend to get dragged into the phosphate density when I do real space
refinement. I have tried playing about with the weight matrix in the
R/RC menu, but this doesn’t seem to help much.
Sphere refinement - this is the sort of thing it was made for...
I am running version 0.6 for Mac (Miramar revision 2540).
Not sure if 0.6 has sphere refinement
Paul.
