On Thu, Sep 22, 2011 at 6:29 AM, Swastik Phulera <[email protected]>wrote:
> I want to have a model of a chimeric protein having domains from two > different proteins(of known structure) . I now have structures of the > individual domains with me. How do I use coot to join these two domian > structures to get one model of my own structure 1. Load molecule "A" into Coot. 2. Load molecule "B" into Coot. 3. From the "Calculate" menu, select "Change chain IDs", choose the file for molecule "B", and set the chain ID to be the same as for molecule "A". 4. For both molecules: from the "Calculate" menu, select "Renumber residues", choose the appropriate molecule, and set the offset as needed for the sequence register to be correct in the chimeric protein. 5. From the "Calculate" menu, select "Merge molecules", and append molecule "B" to molecule "A". 6. Fill in gap between domains as necessary (optional). 7. Save molecule "A" to a new PDB file - it should contain both domains with their proper numbering. There is probably another way to do it, but this seemed like the most obvious method. -Nat
