On 17/01/12 12:01, Takaaki Fukami wrote:
> Dear Coot experts,
>
> I have a protein with an N-acetyl group at its N-terminal, and 
> want to refine it with coot & refmac5.
> Refmac recognize the LINK and applied restraints in 
> $CLIB/list/mon_lib_list.cif.
>
> In Coot, when I "refine zone" from B0 to B1, the ACE group moved away 
> from the next PHE. They won't connect each other.

Try sphere refine, that has more sophisticated linking rules.

>
> I extracted the data_link_ACE_C-N section from the mon_lib_list.cif file
> and import it into Coot, but it didn't work.
>
> How do I include the link restraints into Coot?
>

It should Just Work. If it doesn't then I need to fix it. I've added
this to my list.

LINKRs for refinement are scheduled for 0.8.

Regards,

Paul.

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