And the resolution of your data is... ?
On Mon, 2012-03-26 at 19:58 +0100, Alok Shenoy wrote:
> Hello,
>
> I'm working on a structure using coot, and I have a peculiar problem.
> Searching www didn't quite provide me with an answer, hence requesting more
> knowledgeable users for some help.
>
> Background:
> The structure has 2 chains, Chain A and Chain B. Chain B has all the residues
> that should be present according to our sequence, and the numbering on both
> chains are complete.
>
> Problem:
> 1) Chain A has a couple of residues missing. The numbering shows 231 232 234.
> Clearly marking out the spot where the residue is missing. Sequence shows
> that there is an aspartic acid missing in position 233. Problem is - both 232
> and 234 are connected quite normally, inserting a terminal residue at 232
> (alanine at first) results in absolutely weird connections. Deleting specific
> atoms in the backbone on the protein at that position (between 232 and 234)
> don't quite help either - as that results in 234 missing a peptide bond.
>
> 2) At some points, there is no backbone -by which I mean - lets say residue
> 100 and 101 side chains are showing up (and have appropriate electron
> density). What is missing is the peptide bond that connects these two
> residues (again - electron density for the backbone is present). Is there a
> way to fix this?
>
> Can someone walk me through the solution to this problems?
>
>
> Thank You,
>
> Alok Shenoy
--
Oh, suddenly throwing a giraffe into a volcano to make water is crazy?
Julian, King of Lemurs
